Antioxidant activity using the cuprac method and determination of total phenolate content in the extract and fraction of macroalgae Eucheuma cottonii

Authors

  • Dewi Kurnia Department of Pharmacy, Faculty of Pharmacy, Universitas Bhakti Kencana
  • Desita Rohmah Department of Pharmacy, Faculty of Pharmacy, Universitas Bhakti Kencana
  • Vina Juliana Anggraeni Department of Pharmacy, Faculty of Pharmacy, Universitas Bhakti Kencana

DOI:

https://doi.org/10.31764/jau.v9i4.11116

Keywords:

antioxidant, CUPRAC, Eucheuma cottoni, phenol.

Abstract

Antioxidants are compounds that can counteract free radicals. The macroalgae Euchema cottonii belongs to a type of seaweed from the Rhodophyta which is thought to have antioxidant activity. In this study, the antioxidant activity was tested using the CUPRAC method using spectrophotometry at 450 nm and the determination of total phenolic compounds in the extract and macroalgae fraction Eucheuma cottonii using the Folin Ciocalteu method. The extraction method was carried out using the maceration method with 70% ethanol and fractionation using ECC with n-hexane, ethyl acetate, and methanol-water (20% v/v). The results of TLC monitoring of Eucheuma cottonii showed the presence of flavonoid compounds, phenols, alkaloids, steroids, and triterpenoids. The results of the antioxidant activity test showed that the EC50 for the ethanol extract and the n-hexane, ethyl acetate, and methanol-water fractions were 89.11; 143.40; 100.02 and 90.22 g/ml respectively with EC50 vitamin C as a comparison of 6,86 g/ml. The results of the determination of the total phenolate content of the ethanol extract and the fractions of n- hexane, ethyl acetate, and methanol-water were 2.77; 1.96; 2.76; and 3.09 mg/GAE per 100 mg extract. The ethanol extract had a strong antioxidant activity with an EC50  89.11 g/ml and the highest total phenol content was in the methanol-water fraction of 3.09 mg/GAE per 100 mg extract. The results showed that the ethanolic extract of the macroalga Eucheuma cottonii has the potential as an alternative source of antioxidants.

References

Alicic, R. Z., Rooney, M. T., & Tuttle, K. R. (2017). Diabetic kidney disease: Challenges, progress, and possibilities. Clinical Journal of the American Society of Nephrology, 12(12), 2032–2045. https://doi.org/10.2215/CJN.11491116

Apak, R., Güçlü, K., Özyürek, M., & Karademir, S. E. (2004). Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal of Agricultural and Food Chemistry, 52(26), 7970–7981. https://doi.org/10.1021/jf048741x

Farmakope Herbal Indonesia (II). (2017). Jakarta: Kementrian Kesehatan Republik Indonesia.

Fathmawati D, Abidin MRP, Roesyadi A. 2014. Studi Kinetika Pembentukan Karaginan Dari Rumput Laut. Jurnal Teknik Pomits. Vol 3(1): 27-32.

Forestryana, D., & Arnida, A. (2020). Skrining Fitokimia Dan Analisis Kromatografi Lapis Tipis Ekstrak Etanol Daun Jeruju (Hydrolea Spinosa L.). Jurnal Ilmiah Farmako Bahari, 11(2), 113. https://doi.org/10.52434/jfb.v11i2.859

Gusnedi, R. (2013). Analisis Nilai Absorbansi dalam Penentuan Kadar Flavonoid untuk Berbagai Jenis Daun Tanaman Obat. Pillar of Physics, 2, 76–83.

Hanapi A, Fasya AG, Mardiyah U, Miftahurrahmah. 2013. Aktivitas antioksidan dan antibakteri ekstrakm etanol alga merah Eucheuma spinosum dari perairan Wongsorejo Banyuwangi. Alchemy. Vol 2(2): 126-137.

Kurnia, Dewi., Suhardiman, Aris., Nurdiansyah, Hedy., Ghazhali, M., (2022) Antibacterial Activity Of Eucheuma Cottoni Extract And Fraction Against Acne-Causing Bacteria. Jurnal Agrotek UMMAT, 9(2), 88-94.

Maryam, S., Pratama, R., Effendi, N., & Naid, T. (2016). ANALISIS AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOLIK DAUN YODIUM (Jatropha multifida L.) DENGAN METODE CUPRIC ION REDUCING ANTIOXIDANT CAPACITY (CUPRAC). Jurnal Fitofarmaka Indonesia, 2(1), 90–93. https://doi.org/10.33096/jffi.v2i1.185

Rasmussen, R. S., & Morrissey, M. T. (2007). Marine Biotechnology for Production of Food Ingredients. Advances in Food and Nutrition Research, 52(06), 237–292. https://doi.org/10.1016/S1043-4526(06)52005-4

Sedjati, S., Suryono, S., Santosa, A., Supriyantini, E., & Ridlo, A. (2017). Aktivitas Antioksidan dan Kandungan Senyawa Fenolik Makroalga Coklat Sargassum sp. Jurnal Kelautan Tropis, 20(2), 124. https://doi.org/10.14710/jkt.v20i2.1737

Sharo, N. M., Ningsih, R., Hanapi, A., & Nasichuddin, A. (2013). UJI TOKSISITAS DAN IDENTIFIKASI SENYAWA EKSTRAK ALGA MERAH (Eucheuma cottonii) TERHADAP LARVA UDANG Artemia salina LEACH. Alchemy, 2(3). https://doi.org/10.18860/al.v0i0.2892

Sridharan, M. C., & Dhamotharan, R. (2012). Antibacterial activity of marine brown alga Turbinaria conoides. Journal of Chemical and Pharmaceutical Research, 2012(4), 2292–2294.

Suryanto, E., & Wehantouw, F. (2009). Aktivitas Penangkap Radikal Bebas dari Ekstrak Fenolik Daun Sukun (Artocarpus altilis F.). Chemistry Progress, 2(1), 1–7.

Toga Nugraha, A. (2017). Profil Senyawa Dan Aktifitas Antioksidan Daun Yakon (Smallanthus Sonchifolius) Dengan Metode Dpph Dan Cuprac. Jurnal Ilmiah Farmasi, 13(1), 15–18. https://doi.org/10.20885/jif.vol13.iss1.art3

Yanuarti, R., Nurjanah, N., Anwar, E., & Hidayat, T. (2017). Profil Fenolik dan Aktivitas Antioksidan dari Ekstrak Rumput Laut Turbinaria conoides and Eucheuma cottonii. Jurnal Pengolahan Hasil Perikanan Indonesia, 20(2), 230.

Published

2022-10-31

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Section

Articles