ISOLASI LIKOPEN DARI BUAH TOMAT (LYCOPERSICUM ESCULENTUM) DENGAN PELARUT HEKSANA

Authors

  • Earlyna Sinthia Dewi Teknologi Hasil Pertanian, Universitas Muhammadiyah Mataram

DOI:

https://doi.org/10.31764/agrotek.v5i2.707

Keywords:

Isolasi, Likopen, Lycopersicum esculentu

Abstract

Penelitian ini bertujuan untuk mengembangkan metode sederhana dan efektif  dengan menggunakan ekstraksi pelarut heksana dan methanol sebagai anti pelarut untuk isolasi likopen dari buah tomat (Lycopersicum esculentum). Tomat merupakan  sumber utama likopen. Likopen adalah  zat pigmen golongan karotenoid yang menyebabkan warna merah pada tomat. Penelitian ini telah berhasil mengisolasi likopen dengan metode maserasi. Hasil penelitian menunjukkan dengan pelarut heksana kadar likopen yang diperoleh 2,25 mg/100 g. Analisa gugus fungsi dengan menggunakan spektroskopi Fourier Transform Infra Red (FT-IR) terdeteksi gugus C=C pada panjang gelombang 1537,09 cm-1, gugus CH2 terdeteksi pada panjang gelombang 1498,86 cm-1, gugus R-CH=CH-R terdeteksi pada panjang gelombang 959,27 cm-1. Sedangkan gugus regangan C-C dan C-C-H (streching)masing-masing terjadi pada panjang gelombang 1262,15 cm-1 dan 1378,71 cm-1.

References

Aghel N, Ramezani Z dan Amir Fakhrian S. 2011 “Isolation and Quantification of Lycopene From Tomato Cultivater in Dezfoul, Iranâ€, Jundishapur Journal of Natural Pharmaceutical Products, 6(1), 9-15.

Bunghez I.R, et al, 2011., Lycopene Determination in Tomatoes By Different Spectral Techniques (Uv Vis, FTIR and HPLC), Digest Journal of Nanomaterials and Biostructure, 6(3), 1349-1356.

Cadoni E., De Giorgi M. R., Medda E. and Poma G. 2000. Supercritical CO2 extraction of lycopene and β-carotene from ripe tomatoes. Dyes and Pigments 44:27-32.

Davies, J. 2000. Tomatoes and Health. Journal of Social Health. June : 120(2): 81-82

Hakala S.H. and Heinonen I.M. 1994. Chromatographic purifi - cation of natural lycopene. Journal of Agricultural and Food Chemistry 42:1314-1316.

Kamil Mohle, et al, 2011. Fourier Transformer Infrared Spectroscopy for Quality Assurance of Tomato Products, Journal of American Science, 7(6), 559-572.

Liu B., Huang X.Y., Di D.L. and Zhang B.C. 2008. Study on quality standard for lycopene soft gels. Food Science and Technology 34:243-245.

Myong Roh, Kyun, Min Hee Jeon, Jin Nam Moon, Woi Sook Moon, Sun Mee Park dan Jae Suk Choi, A Simple Method For The Isolation of Lycopene from Lycopersicon Esculentum, Botanical Sciences, 91 (2), 187-192, 2013

Nutrient Data Laboratory, ARS, USDA National Food and Nutrient Analysis Program Wave, Beltsville MD, 2001

Rao, V. A. dan Agarwal, S. 2000, Role of Antioidant Lycopene in Cancer and Heart Disease, J. American College of Nutrition, 19(5): 563-569.

Rizk, et al. 2014. Charactrization of carotenoids (lyco-red) extracted from tomato peels and its uses as natural colorants and antioxidants of ice cream. Annals of Agricultural Science. Vol. 59. Pp: 53-61

Sadler G., Davis J. and Dezman D. 1990. Rapid extraction of lycopene and β-carotene from reconstituted tomato paste and pink grapefruit homogenates. Journal of Food Science 55:1460-1461.

O’Neill, M. J., 2006. The Merck Index An Encyclopedia of Chemical, Drugs and Biologicals. 14th Edition, N.J., USA: Merck & Co., Inc., 630, 974-975 dan 6973

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Published

2018-08-30

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